Abstract
The review presents an analysis of the chemical chemicoenzymic, and enzymic methods of synthesis of nucleoside 5'-triphosphates labelled with phosphorus isotopes ( 32P and 33P) in the α- and γ-positions. The major part of the review is devoted to enzymic methods of synthesis, because they have undoubted advantages. The enzymatic reactions are described in detail, the specificity and availability of the enzymes are considered, and data on the radiation stability of certain enzymes are presented. The bibliography includes 29 references.
References
1.
Evans M.R., Read C.A.
Nature ,
1992
2.
Southern E.M.
Journal of Molecular Biology ,
1975
3.
Biggin M.D., Gibson T.J., Hong G.F.
Proceedings of the National Academy of Sciences of the United States of America ,
1983
4.
Murray V.
Nucleic Acids Research ,
1989
5.
Orita M., Suzuki Y., Sekiya T., Hayashi K.
Genomics ,
1989
6.
Frank-Kamenetskaya M.D., Knizhnikova G.V., Myasoyedov N.F.
Journal of Radioanalytical and Nuclear Chemistry ,
1988
7.
Moffatt J.G.
Methods in Enzymology ,
1967
8.
8
V. N. Kondrat'ev
"Kinetika Reaktsii v Gazovoi Faze" (Kinetics of Reactions in the Gas Phase) ,
1959
9.
9
V. H. Lynch
Carnegie Inst. Year Book ,
1954
10.
A simple method for the preparation of 32P-labelled adenosine triphosphate of high specific activity
Glynn I., Chappell J.
Biochemical Journal ,
1964
11.
Maxam A.M., Gilbert W.
Methods in Enzymology ,
1980
12.
12
V. B. Evstigneev
Dokl. Akad. Nauk SSSR ,
1950
13.
Schendel P.F., Wells R.D.
Journal of Biological Chemistry ,
1973
14.
Walseth T.F., Johnson R.A.
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis ,
1979
15.
15
A. S. Holt
"Research in Photosynthesis" ,
1957
16.
16
A. A. Krasnovskii
Dokl. Akad. Nauk SSSR ,
1949
17.
17
V. B. Evstigneev
Dokl. Akad. Nauk SSSR ,
1958
18.
18
A. A. Krasnovskii
Dokl. Akad. Nauk SSSR ,
1951
19.
Symons R.H.
Methods in Enzymology ,
1974
20.
20
A. A. Krasnovskii
Dokl. Akad. Nauk SSSR ,
1954
21.
21
A. A. Krasnovskii
Dokl. Akad. Nauk SSSR ,
1958
22.
Biebricher C.K.
Analytical Biochemistry ,
1979
23.
Hurlbert R.B., Furlong N.B.
Methods in Enzymology ,
1967
24.
24
M. S. Ashkinazi
Dokl. Akad. Nauk SSSR ,
1951
25.
25
G. W. Robinson
J. Chem. Phys. ,
1955
26.
26
B. Ya. Dain
1957
27.
27
A. A. Krasnovskii
Biofizika ,
1956
28.
28
A. A. Krasnovskii
Dokl. Akad. Nauk SSSR ,
1955
29.
Walseth T.F., Yuen P.S., Moos M.C.
Methods in Enzymology ,
1991